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Description
SPARC Recombinant Rabbit mAb (SDT-R136)Product Specification Host Rabbit Antigen SPARC Synonyms BM 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine Immunogen N A Location Secreted, Extracellular matrix, Basement membrane Accession P09486 Clone Number SDT R136 Application WB, IHC P, IP Reactivity Hu Purification Protein A Concentration 0. 5 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from
Product Specification
| Host | Rabbit |
| Antigen | SPARC |
| Synonyms | BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine |
| Immunogen | N/A |
| Location | Secreted, Extracellular matrix, Basement membrane |
| Accession | P09486 |
| Clone Number | SDT-R136 |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:2000 | null |
| IP | 1:50 | null |
Background
SPARC, also referred to as osteonectin or basement membrane protein 40 (BM-40), is a ~32 kDa calcium-binding matricellular protein [PMID: 22249026, PMID: 25064829]. At one time SPARC was thought to be expressed specifically in mineralized tissues (thus the name osteonectin) but was subsequently demonstrated to have a much wider expression pattern in both mineralized and non-mineralized tissues [PMID: 7034958]. Typically, expression of SPARC is closely aligned with that of fibrillar collagens such as collagen type I. The protein component of the pre-mineralized bone matrix (osteoid) is composed mainly of collagen type I (94%) and a number of noncollagenous proteins.
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